STR-typing of ancient skeletal remains: which multiplex-PCR kit is the best?
نویسندگان
چکیده
منابع مشابه
STR-typing of ancient skeletal remains: which multiplex-PCR kit is the best?
AIM To comparatively test nine co mmercially available short tandem repeat (STR)-multiplex kits (PowerPlex 16, 16HS, ES, ESI17, ESX17, S5 [all Promega]; AmpFiSTR Identifiler, NGM and SEfiler [all Applied Biosystems]) for their efficiency and applicability to analyze ancient and thus highly degraded DNA samples. METHODS Fifteen human skeletal remains from the late medieval age were obtained an...
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AIM To evaluate the performance of three multiplex short tandem repeat (STR) systems (AmpflSTR Profiler, AmpflSTR Profiler Plus, and AmpflSTR COfiler), and a megaplex STR system (PowerPlex 16) on DNA extracted from the skeletal remains. By performing a microbial DNA challenge study, we also evaluated the influence of microbial DNA on human DNA typing. METHODS A subset of 86 DNA extracts isola...
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ژورنال
عنوان ژورنال: Croatian Medical Journal
سال: 2012
ISSN: 0353-9504,1332-8166
DOI: 10.3325/cmj.2012.53.416